Composite

Part:BBa_K195618

Designed by: Yun-Hsiang Chang   Group: iGEM09_NYMU-Taipei   (2009-10-18)

pPenI with activator gene CII and pRE with GFP generator

This part is composed of pPenI with activator gene CII, and it would induce pRE to produce green fluorescent protein.The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pPenI and produce green fluorescent protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1260


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